Sakagami, M., Ohnishi, Y., Kumaki, Y., Aizawa, T., and Inoue, A. (2023) Enzymatic characterization of a mannuronan C5-epimerase from the subtropical brown alga Cladosiphon okamuranus. Fisheries Sci. 89, 823-835. [Link]

Nishiyama, R., Ojima, T., Ohnishi, Y., Kumaki, Y., Aizawa, T., and Inoue, A. (2021) An oxidative metabolic pathway of 4-deoxy-L-erythro-5-hexoseulose uronic acid (DEHU) from alginate in an alginate-assimilating bacterium. Commun. Biol. 4, 1254. [Link]

Inoue, A., Kudo, M., Werner, E., and Ojima, T. (2021) Identification and characterization of cellouronate (β-1,4-linked polyglucuronic acid) lyase from the scallop Mizuhopecten yessoensis. Carbohydr. Polym. 254, 117306. [Link]

Inoue, A. and Ojima, T. (2021) Functional identification of the 4-deoxy-L-erythro-5-hexoseulose uronate reductase from a brown alga, Saccharina japonica. Biochem. Biophys. Res. Commun. 545, 112–118. [Link]

Arai, K., Inoue, A., and Ojima, T. (2020) Isolation and characterization of an adenylate kinase from the lateral muscle of bastard halibut Paralichthys olivaceus. Fisheries Sci. 86, 385–394. [Link]

Narsico, J., Inoue, A., Oka, S., and Ojima, T. (2020) Production of a novel dimeric 4-deoxy-L-erythro-5-hexoseulose uronic acid by a PL-17 exolytic alginate lyase from Hydrogenophaga sp. UMI-18. Biochem. Biophys. Res. Commun. 525, 982–988. [Link]

Inoue, A. and Ojima, T. (2019) Functional identification of alginate lyase from the brown alga Saccharina japonica. Sci. Rep. 9, 4937. [Link]

Inoue, A., Iwayama, T., and Ojima, T. (2019) Complementary DNA cloning and functional analysis of lycopene β-cyclase in the brown alga Undaria pinnatifida. Fisheries Sci. 85, 717–729. [Link]

Yamaguchi, T., Narsico, J., Kobayashi, T., Inoue, A., and Ojima, T. (2019) Production of poly(3-hydroyxybutylate) by a novel alginolytic bacterium Hydrogenophaga sp. strain UMI-18 using alginate as a sole carbon source. J. Biosci. Bioeng. 128, 203–208. [Link]

Qin, H.-M., Miyakawa, T., Inoue, A., Nishiyama, R., Nakamura, A., Asano, A., Ojima, T., and Tanokura, M. (2018) Structural basis for controlling the enzymatic properties of polymannuronate preferred alginate lyase FlAlyA from the PL-7 family. Chem. Commun. 54, 555–558. [Link]

Ojima, T., Rahman, M. M., Kumagai, Y., Nishiyama, R., Narsico, J., and Inoue, A. (2018) Polysaccharide-degrading enzymes from marine gastropods. Methods Enzymol. 605, 457–497. [Link]

Inoue, A. (2018) Characterization of PL-7 family alginate lyases from marine organisms and their applications. Methods Enzymol. 605, 499–524. [Link]

Mitsuya, D., Yamamoto, M., Okai, M., Inoue, A., Suzuki, T., Ojima, T., and Urano, N. (2017) Continuous saccharification of laminarin by immobilized laminarinase Ulam111 followed by ethanol fermentation with a marine-derived yeast. Adv. in Microbiol. 7, 387–403. [Link]

Qin, H.-M., Miyakawa, T., Inoue, A., Nishiyama, R., Nakamura, A., Asano, A., Sawano, Y., Ojima, T., and Tanokura, M. (2017) Structure and polymannuronate specificity of a eukaryotic member of polysaccharide lyase family 14. J. Biol. Chem. 292, 2182–2190. [Link]

Nishiyama, R., Inoue, A., and Ojima, T. (2017) Identification of 2-keto-3-deoxy-D-gluconate kinase and 2-keto-3-deoxy-D-phosphogluconate aldolase in an alginate-assimilating bacterium, Flavobacterium sp. strain UMI-01. Mar. Drugs 15, 37. [Link]

Qin, H.-M., Miyakawa, T., Inoue, A., Nakamura, A., Nishiyama, R., Ojima, T., and Tanokura, M. (2017) Laminarinase from Flavobacterium sp. reveals the structural basis of thermostability and substrate specificity. Sci. Rep. 7, 11425. [Link]

Inoue, A., Satoh, A., Morishita, M., Tokunaga, Y., Miyakawa, T., Tanokura, M., and Ojima, T. (2016) Functional heterologous expression and characterization of mannuronan C5-epimerase from the brown alga Saccharina japonica. Algal Res. 16, 282–291. [Link]

Inoue, A., Anraku, M., Nakagawa, S., and Ojima, T. (2016) Discovery of a novel alginate lyase from Nitratiruptor sp. SB155-2 thriving at deep-sea hydrothermal vents and identification of the residues responsible for its heat stability. J. Biol. Chem. 291, 15551–15563. [Link]

Inoue, A., Nishiyama, R., and Ojima, T. (2016) The alginate lyases FlAlyA, FlAlyB, FlAlyC, and FlAlex from Flavobacterium sp. UMI-01 have distinct roles in the complete degradation of alginate. Algal Res. 19, 355–362. [Link]

Terauchi, M., Nagasato, C., Inoue, A., Ito, T., and Motomura, T. (2016) Distribution of alginate and cellulose and regulatory role of calcium in the cell wall of the brown alga Ectocarpus siliculosus (Ectocarpales, Phaeophyceae). Planta 244, 361–377. [Link]

Inoue, A., Mashino, C., Uji, T., Saga, N., Mikami, K., and Ojima, T. (2015) Characterization of an eukaryotic PL-7 alginate lyase in the marine red alga Pyropia yezoensis. Curr. Biotechnol. 4, 240–248. [Link]

Inoue, A., Nishiyama, R., Mochizuki, S., and Ojima, T. (2015) Identification of a 4-deoxy-L-erythro-5-hexoseulose uronic acid reductase, FlRed, in an alginolytic bacterium Flavobacterium sp. strain UMI-01. Mar. Drugs 13, 493–508. [Link]

Mochizuki, S., Nishiyama, R., Inoue, A., and Ojima, T. (2015) A novel aldo-keto reductase, HdRed, from the pacific abalone Haliotis discus hannai, which reduces alginate-derived 4-deoxy-L-erythro-5-hexoseulose uronic acid to 2-keto-3-deoxy-D-gluconate. J. Biol. Chem. 290, 30962–30974. [Link]

Rahman, M. M., Inoue, A., and Ojima, T. (2014) Characterization of a GHF45 cellulase, AkEG21, from the common sea hare Aplysia kurodai. Front. Chem. 2, 60. [Link]

Kumagai, Y., Satoh, T., Inoue, A., and Ojima, T. (2014) A laminaribiose-hydrolyzing enzyme, AkLab, from the common sea hare Aplysia kurodai and its transglycosylation activity. Comp. Biochem. Physiol. B, Biochem. Mol. Biol. 167, 1–7. [Link]

Inoue, A., Takadono, K., Nishiyama, R., Tajima, K., Kobayashi, T., and Ojima, T. (2014) Characterization of an alginate lyase, FlAlyA, from Flavobacterium sp. strain UMI-01 and its expression in Escherichia coli. Mar. Drugs 12, 4693–4712. [Link]

Kumagai, Y., Satoh, T., Inoue, A., and Ojima, T. (2013) Enzymatic properties and primary structures of two α-amylase isozymes from the Pacific abalone Haliotis discus hannai. Comp. Biochem. Physiol. B, Biochem. Mol. Biol. 164, 80–88. [Link]

Ueda, T., Nakamura, Y., Smith, C. M., Copits, B. A., Inoue, A., Ojima, T., Matsunaga, S., Swanson, G. T., and Sakai, R. (2013) Isolation of novel prototype galectins from the marine ball sponge Cinachyrella sp. guided by their modulatory activity on mammalian glutamate-gated ion channels. Glycobiology 23, 412–425. [Link]

Satoh, T., Inoue, A., and Ojima, T. (2013) Characterization of an α-glucosidase, HdAgl, from the digestive fluid of Haliotis discus hannai. Comp. Biochem. Physiol. B, Biochem. Mol. Biol. 166, 15–22. [Link]

Zahura, U. A., Rahman, M. M., Inoue, A., and Ojima, T. (2012) Characterization of a β-D-mannosidase from a marine gastropod, Aplysia kurodai. Comp. Biochem. Physiol. B, Biochem. Mol. Biol. 162, 24–33. [Link]

Wang, L., Rahman, M. M., Inoue, A., and Ojima, T. (2012) Heat-stability and primary structure of the major alginate lyase isozyme LbAly35 from Littorina brevicula. Fisheries Sci. 78, 889–896. [Link]

Rahman, M. M., Wang, L., Inoue, A., and Ojima, T. (2012) cDNA cloning and bacterial expression of a PL-14 alginate lyase from a herbivorous marine snail Littorina brevicula. Carbohydr. Res. 360, 69–77. [Link]

Inoue, A., Mashino, C., Kodama, T., and Ojima, T. (2011) Protoplast preparation from Laminaria japonica with recombinant alginate lyase and cellulase. Mar. Biotechnol. 13, 256–263. [Link]

Zahura, U. A., Rahman, M. M., Inoue, A., Tanaka, H., and Ojima, T. (2011) cDNA cloning and bacterial expression of an endo-β-1,4-mannanase, AkMan, from Aplysia kurodai. Comp. Biochem. Physiol. B, Biochem. Mol. Biol. 159, 227–235.

Rahman, M. M., Inoue, A., Tanaka, H., and Ojima, T. (2011) cDNA cloning of an alginate lyase from a marine gastropod Aplysia kurodai and assessment of catalytically important residues of this enzyme. Biochimie 93, 1720–1730.

Nagasato, C., Inoue, A., Mizuno, M., Kanazawa, K., Ojima, T., Okuda, K., and Motomura, T. (2010) Membrane fusion process and assembly of cell wall during cytokinesis in the brown alga, Silvetia babingtonii (Fucales, Phaeophyceae). Planta 232, 287–298.

Zahura, U. A., Rahman, M. M., Inoue, A., Tanaka, H., and Ojima, T. (2010) An endo-β-1,4-mannanase, AkMan, from the common sea hare Aplysia kurodai. Comp. Biochem. Physiol. B, Biochem. Mol. Biol. 157, 137–143.

Rahman, M. M., Inoue, A., Tanaka, H., and Ojima, T. (2010) Isolation and characterization of two alginate lyase isozymes, AkAly28 and AkAly33, from the common sea hare Aplysia kurodai. Comp. Biochem. Physiol. B, Biochem. Mol. Biol. 157, 317–325.

Hata, M., Kumagai, Y., Rahman, M. M., Chiba, S., Tanaka, H., Inoue, A., and Ojima, T. (2009) Comparative study on general properties of alginate lyases from some marine gastropod mollusks. Fisheries Sci. 75, 755–763.

Kumagai, Y., Inoue, A., Tanaka, H., and Ojima, T. (2008) Preparation of β-1,3-glucanase from scallop mid-gut gland drips and its use for production of novel heterooligosaccharides. Fisheries Sci. 74, 1127–1136.

Yamamoto, S., Sahara, T., Sato, D., Kawasaki, K., Ohgiya, S., Inoue, A., and Ojima, T. (2008) Catalytically important amino-acid residues of abalone alginate lyase HdAly assessed by site-directed mutagenesis. Enzyme Microb. Technol. 43, 396–402.

Inoue, A., Kagaya, M., and Ojima, T. (2007) Preparation of protoplasts from Laminaria japonica using native and recombinant abalone alginate lyases. J. Appl. Phycol. 20, 633–640.

Nishida, Y., Suzuki, K.-I., Kumagai, Y., Tanaka, H., Inoue, A., and Ojima, T. (2007) Isolation and primary structure of a cellulase from the Japanese sea urchin Strongylocentrotus nudus. Biochimie 89, 1002–1011.

Suzuki, H., Suzuki, K.-I., Inoue, A., and Ojima, T. (2006) A novel oligoalginate lyase from abalone, Haliotis discus hannai, that releases disaccharide from alginate polymer in an exolytic manner. Carbohydr. Res. 341, 1809–1819.

Ootsuka, S., Saga, N., Suzuki, K.-I., Inoue, A., and Ojima, T. (2006) Isolation and cloning of an endo-β-1,4-mannanase from Pacific abalone Haliotis discus hannai. J. Biotechnol. 125, 269–280.

Doi, T., Satoh, A., Tanaka, H., Inoue, A., Yumoto, F., Tanokura, M., Ohtsuki, I., Nishita, K., and Ojima, T. (2005) Functional importance of Ca²⁺-deficient N-terminal lobe of molluscan troponin C in troponin regulation. Arch. Biochem. Biophys. 436, 83–90.

Inoue, A., Ojima, T., and Nishita, K. (2004) N-Terminal modification and its effect on the biochemical characteristics of Akazara scallop tropomyosins expressed in Escherichia coli. J. Biochem. 136, 107–114.